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1x nebuffer tm r3 1  (New England Biolabs)


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  • 99

    Structured Review

    New England Biolabs 1x nebuffer tm r3 1
    1x Nebuffer Tm R3 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+nebuffer+tm+r3+1/NEBuffer+r3%2E1/pmc12362242-83-31-32
    Average 99 stars, based on 275 article reviews
    1x nebuffer tm r3 1 - by Bioz Stars, 2026-09
    99/100 stars

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    Recombinant:

    Article Title: Kingdom-wide CRISPR guide design with ALLEGRO
    Article Snippet: .. Cas9–RNPs were immediately assembled before experiments in a 50 μl reaction in buffer B (25 mM CaCl 2 , 10 mM Tris–HCl, 1 M sorbitol, pH 7.5) containing 5 μl of 1X NEBuffer TM r3.1 (100 mM NaCl, 50 mM Tris–HCl, 10 mM MgCl 2 , and 100 μg/mL Recombinant Albumin, pH 7.9), and 1:1 molar ratio of sgRNA:Cas9 at 37 °C water bath for 10 min. .. We instructed ALLEGRO to design an sgRNA library with minimal size targeting a counter-selectable marker gene set ( S. cerevisiae orthologs of CAN1 , FCY1 , GAP1 , LYS2 , TRP1 , and URA3 ) across 2263 fungal species using track E 1 , meaning that each gene in the set must be targeted at least once in each species (list of genes in , and list of species in ).



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    99
    New England Biolabs 1x nebuffer tm r3 1
    1x Nebuffer Tm R3 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+nebuffer+tm+r3+1/NEBuffer+r3%2E1/pmc12362242-83-31-32
    Average 99 stars, based on 1 article reviews
    1x nebuffer tm r3 1 - by Bioz Stars, 2026-09
    99/100 stars
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